Quantitative determination of trans- 10-Hydroxy-2-Decenoic Acid (10-HDA) in Brazilian royal jelly and commercial products containing royal jelly | Determinación cuantitativa del ácido trans-10-hidroxi-2-decenoico (10-HDA) en jalea real brasileña y en productos comerciales conteniendo jalea real
2007
Caparica-Santos, Celira | Marcucci, Maria Cristina
We report the analysis of Brazilian commercial bee products using high performance liquid chromatography, and a faster method, to detect trans-10-Hydroxy-2-Decenoic Acid or 10-Hydroxy-2-E-decenoic acid (10-HDA) in royal jelly. Determination was carried out using aqueous phosphoric acid 0.1% (pH 2.5)/methanol (50/50) as the mobile phase, and a LiChrospher® RP-18 column with detection at 215 nm. A good linearity was shown between the concentration of 10-HDA and peak area in the concentration range of 5.0–50.0 mg/L (r 0.99, n = 5). The within-day and between-day relative standard deviation (RSD) were1 % and 3 %, respectively.
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