Der Einfluß von Cycloheximid und Chloramphenicol auf die Biosynthese der Photosynthese-Pigmente in Cyanophora paradoxa Korsch. II. carotinoide / The influence of cycloheximide and chloram phenicol on the biosynthesis of the photosynthetic pigments in cyanophora paradoxa. II. carotenoids
2014
Neu-Müller, Margarete | Schenk, Hainfried E. A. | Stransky, Harald
To prove the extent to which the endocyanelle of C. paradoxa is autonomous with respect to the biosynthesis of its photosynthetic pigments, the degree, to which the translation inhibitors cycloheximide (CHI) and chloramphenicol (CA) influence the biosynthesis of carotenoids, was investigated by two methods (in vivo VIS-spectroscopy and photosynthetic ˡ⁴C-incorporation into the pigments). During the first 6 h (in vivo VIS-spectra) the probes treated with the inhibitors show a similar increase of the total carotenoid content as the control, thereafter the inhibition by CHI is increased significantly at approx. 50% (18 h), by CA somewhat lower. The [¹⁴C]carbon- dioxide incorporation into β-carotene do not show any notable differences between the control culture and the cultures treated with inhibitors. Quite different to this observation is the behaviour of zeaxanthin. CHI shows an inhibition of 60% after 8 h, whereas CA accelerates the biosynthesis of zeaxanthin at +60% (to the control). Zeaxanthin has the same absolute concentration, but it was marked only ten times lower than β-carotene. The greater effect of CHI (in vivo VIS-spectra) should not be taken as a higher participation of the eukaryotic genome. In contrast that is only to ascribe to the inhibition of the photosynthetic activity, which is in the same order of magnitude. Additionally the behaviour of zeaxanthin seems to be more a sign for higher participation of the prokaryotic genome
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