Response surface methodology in media optimization for production of β-carotene from Daucus carota
2008
Hanchinal, V. M | Survase, S. A | Sawant, S. K | Annapure, U. S
Plants are a valuable source of a vast array of chemical compounds including fragrances, flavours, food additives, colours, natural sweeteners, industrial feedstocks, anti-microbials and pharmaceuticals. The present study reports on application of Response Surface Methodology (RSM) in media optimization for suspension culture for the production of β-carotene. Growth kinetics of carrot cells in suspension culture has been carried out to understand the relationship between growth and β-carotene formation. The maximum production of β-carotene obtained using the optimized medium was 13.614 μg/g dry weight cell mass. The μ (specific growth rate) and t d (doubling time) were found to be higher for 20 g DW/l inoculum size.
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