A pyrene-based highly selective turn-on fluorescent sensor for copper(II) ions and its application in living cell imaging
2013
Wang, Hsuan-Fu | Wu, Shu-Pao
A new pyrene derivative (1) containing a benzothiazolenhydrazone moiety exhibited high selectivity for Cu²⁺ detection. In the presence of Cu²⁺, chemosensor 1 provided significant fluorescence enhancement, while Ag⁺, Ca²⁺, Cd²⁺, Co²⁺, Fe²⁺, Fe³⁺, Hg²⁺, K⁺, Mg²⁺, Mn²⁺, Ni²⁺, Pb²⁺, and Zn²⁺ metal ions produced only minor changes in fluorescence intensity. The association constant (Kₐ) for Cu²⁺ binding to 1 had a value of 5.00×10⁸M⁻². The maximum fluorescence enhancement induced by Cu²⁺ binding to the chemosensor was observed over the range pH 2–8.5. Our fluorescence microscopy experiments demonstrate that chemosensor 1 may have application as a fluorescent probe for detecting Cu²⁺ in living cells.
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