Antioxydant response to biotic and abiotic inducers for the resistance against fusarium wilt disease in eggplant (solanum melongena l.)
2014
Altinok, Hacer H. | Dikilitas, Murat
Acibenzolar-S-methyl as an abiotic plant activator and a non-host isolate of Fusarium oxysporum on eggplant (F. oxysporum f. sp. melonis) as a biotic inducer were applied to eggplant seedlings in order to confer increased resistance to F. oxysporum f. sp. melongenae, the causal agent of Fusarium wilt of eggplant. Acibenzolar-S-methyl and F. oxysporum f. sp. melonis were applied 72 h before pathogen inoculation and the development of disease symptoms was assessed with a Fusarium yellow rating at 7th, 11th, 14th, 17th and 21th day after inoculation. Pretreatment of eggplants with Acibenzolar-S-methyl and F. oxysporum f. sp. melonis significantly reduced the severity of Fusarium wilt disease. The severity of the disease in positive control plants reached to 92.50% whereas that of acibenzolar-S-methyl and F. oxysporum f. sp. melonis-pretreated seedlings of eggplants was only 32.21% and 21.13%, respectively, 21 days after inoculation. Acibenzolar-S- -methyl and F. oxysporum f. sp. melonis pretreatments resulted in a hypersensitive reaction and triggered the elaboration of histological barriers such as callose and H₂O₂ synthesis. In situ studies demonstrated that the hydrogen peroxide (H₂O₂) accumulation and the callose deposition as responses to the pathogen attack started 24 h after inoculation. Acibenzolar- S-methyl and F. oxysporum f. sp. melonis-pretreated plants also showed significant increases in the activity of catalase and polyphenol oxidase enzymes along with the increase of proline and H₂O₂ content when compared to F. oxysporum f. sp. melongenae-infected plants.
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