How the edaphic Bacillus megaterium strain Mes11 adapts its metabolism to the herbicide mesotrione pressure
2015
Bardot, Corinne | Besse-Hoggan, Pascale | Carles, Louis | Le Gall, Morgane | Clary, Guilhem | Chafey, Philippe | Federici, Christian | Broussard, Cédric | Batisson, Isabelle
Toxicity of pesticides towards microorganisms can have a major impact on ecosystem function. Nevertheless, some microorganisms are able to respond quickly to this stress by degrading these molecules. The edaphic Bacillus megaterium strain Mes11 can degrade the herbicide mesotrione. In order to gain insight into the cellular response involved, the intracellular proteome of Mes11 exposed to mesotrione was analyzed using the two-dimensional differential in-gel electrophoresis (2D-DIGE) approach coupled with mass spectrometry. The results showed an average of 1820 protein spots being detected. The gel profile analyses revealed 32 protein spots whose abundance is modified after treatment with mesotrione. Twenty spots could be identified, leading to 17 non redundant proteins, mainly involved in stress, metabolic and storage mechanisms. These findings clarify the pathways used by B. megaterium strain Mes11 to resist and adapt to the presence of mesotrione.
显示更多 [+] 显示较少 [-]