Effect of different seed storage conditions on germination and isozyme activity in some Brassica species
1995
Ramiro, M.C. | Perez-Garcia, F. | Aguinagalde, I.
Sixteen seed accessions of four Brassica species (B. cretica, B. incana, B. montana and B. oleracea) were examined following 5-22 years' storage in the germplasm bank. Germinability and isozyme activity of seeds stored under long-term (-10 degrees C and 3% moisture content) and short-term (5 degrees C and 8% moisture content) storage conditions were compared. Long-term storage produced no deterioration and the ability to germinate was satisfactorily maintained over 8-22 years. Short-term storage conditions maintained germination ability up to 10-12 years in all accessions of B. cretica and B. montana. However, seed multiplication might be essential every 10 years for some accessions of B. oleracea stored in this way. In the 8 to 9-year-old accessions of B. cretica, B. incana and B. montana, no significant differences were detected between conservation systems for germinability and frequency of seeds showing isozyme activity for seven enzyme systems (ACO, IDH, MDH, ME, PGI, PGM and 6-PGD). However, significant differences were found for the ADH enzyme system. Moreover, in the 5-22-year-old B. oleracea accessions, significant differences were found between storage conditions with respect to isozyme activity for all enzyme systems studied.
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