Food peptides as inducers of CCK and GLP-1 secretion and GPCRs involved in enteroendocrine cell signalling
2023
Santos-Hernández, Marta | Vivanco-Maroto, Santiaga María | Miralles, Beatriz | Recio, Isidra
The strong effect of protein digestion products on gastrointestinal-released hormones is recognised. However, little is known about the specific peptide sequences able to induce gastrointestinal hormone secretion and the receptors involved. Our objective was to identify peptides able to induce the secretion of cholecystokinin (CCK) and glucagon like peptide-1 (GLP-1) in the enteroendocrine cell line STC-1, and to evaluate the involvement of the calcium-sensing receptor and G-protein coupled receptor-93 in this cell signalling. The key role of the amino acidic sequence on CCK and GLP-1 secretion is demonstrated. Removing Ser from the N-terminus of κ-casein ³³SRYPS³⁷, or the N-terminal Trp-Ile in lysozyme ¹²³WIRGCRL¹²⁹ decreased the secretion of both hormones. However, substituting Tyr by Ala in peptide αₛ₁-CN ⁹⁰RYLG⁹³ enhanced the CCK secretion levels but not the GLP-1 ones. In addition, the involvement of CaSR and GPR93 was evidenced, but our results pointed to the contribution of additional receptors or transporters.
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