Inhibition of Photophosphorylation and Electron Transport Chain in Thylakoids by Lasiodiplodin, a Natural Product from Botryosphaeria rhodina
2007
Veiga, T.A.M. | Silva, S.C. | Francisco, A.C. | Filho, E.R. | Vieira, P.C. | Fernandes, J.B. | Silva, M.F.G.F. | Müller, M.W. | Lotina-Hennsen, B.
Four natural products were isolated from the fungus Botryosphaeria rhodina, and their effects on photosynthesis were tested. Only lasiodiplodin (1) inhibited ATP synthesis and electron flow from water to methylviologen; therefore, it acts as a Hill reaction inhibitor in freshly lysed spinach thylakoids. Photosystem I and II and partial reactions as well as ATPase were measured in the presence of 1. Three new different sites of 1 interaction and inhibition were found: one at CF₁, the second in the water-splitting enzyme, and the third at the electron-transfer path between P₆₈₀ and Q(A); these targets are different from that of the synthetic herbicides present. Electron transport chain inhibition by 1 was corroborated by fluorescence induction kinetics studies.
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