Revealing the effect of lipopeptide on improving the probiotics characteristics: Flavor and texture enhancer in the formulated yogurt
2022
Ravindran, Amrudha | Kiran, G Seghal | Selvin, Joseph
Probiotics are beneficial viable microorganisms that protects from pathogens, enhance immunity and health. In this study, a biosurfactant from marine sponge associated bacteria MS48 was used to effectively enhance the survival of starter cultures and thereby improving their functional properties. The biosurfactant MS48 was characterized as lipopeptide based upon the spectroscopic analysis. The GC-MS analysis showed the moiety as l-glutamic acid methyl ester with the mass of m/z 161.16. Probiotics supplemented with lipopeptide showed better survival in the stress tolerance assays includes acid, bile, heat, salt stress and other assays including auto-aggregation, hydrophobicity, microbial adhesion to solvents (MATS), and simulated gastric juice. Yogurt formulated using lipopeptide showed enhanced flavor components, stability, improved characteristics, EPS production, and lower syneresis than the control. Malonic acid, acidity regulator was detected in the mass spectrum of lipopeptide added yogurt. The texture analysis of the lipopeptide added yogurt showed improved textural and sensorial properties when compared to the control.
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