Relative populations and toxin production by Aspergillus flavus and Fusarium verticillioides in artificially inoculated corn at various stages of development under field conditions
2008
Zorzete, Patricia | Castro, Rosimar S. | Pozzi, Claudia R. | Israel, Ana Lia M. | Fonseca, Homero | Yanaguibashi, Gianni | Corrêa, Benedito
The presence, development and production of mycotoxins by Aspergillus flavus and Fusarium verticillioides were studied in corn ears under field conditions after artificial contamination of corn silks. The planted area was divided into five treatments: T1, inoculated with A. flavus solution containing 1 x 10⁸ spores, ears covered; T2, inoculated with F. verticillioides solution containing 1 x 10⁸ spores, ears covered; T3, inoculated with F. verticillioides plus A. flavus solution containing 1 x 10⁸ spores of each, ears covered; T4, sprayed with sterile phosphate-buffered saline, ears covered; T5, non-sprayed silks, uncovered ears. Soil and air samples were also collected and analysed for the occurrence of fungi. Water activity, relative air humidity, rainfall and temperature were determined to assess the correlation between abiotic factors and the presence of fungi in the samples. Contamination with the inoculated fungus predominated in T1 and T2. In the other treatments, F. verticillioides was the most frequently isolated contaminant irrespective of treatment. Considering the production of mycotoxins, a positive relation between the production of fumonisins B₁ and B₂ and the frequency of F. verticillioides was statistically verified in all treatments.
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