Effect of chemical modification of sunflower 11S protein on the binding of chlorogenic acid
1991
Shamanthaka Sastry, M.C. | Narasinga Rao, M.S.
The binding of chlorogenic acid by sunflower 11S protein and succinylated and N-ethylmaleimide-treated protein was measured at ph 4.0 in 0.1 M acetate buffer. Succinylation reduced binding, whereas N-ethylmaleimide treatment did not. Analysis of the binding data showed,that succinylation reduced the number of binding sites without affecting the binding affinity. N-ethylmaleimide treatment reduced neither the number of binding sites nor the binding affinity. Succinylation dissociated the 11S protein, whereas N-ethylmaleimide treatment did not. The secondary structure of N-ethylmaleimide-treated protein was different from that of the unmodified protein.
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