The use of AFLP markers for the identification of carrot breeding lines and F1 hybrids
2001
Grzebelus, D. | Senalik, D. | Jagosz, B. | Simon, P.W. | Michalik, B.
Four inbred lines of carrot (cytoplasmic male-steriles and corresponding maintainers) and eight of their F1 hybrids were studied with the amplified fragment length polymorphism (AFLP) technique to examine their genetic relationship and produce markers useful for testing hybrid seed purity. Eighty-six polymorphic amplicons were identified in bulked DNA samples using eight primer pair combinations. Genetic distance was estimated on the basis of the presence or absence of polymorphic bands. The dendrogram plotted on the basis of the AFLP data closely represented the pedigree relationships of the lines and their hybrids. From one to six amplicons specific for a breeding line were identified. Most of them were also present in the DNA bulks of respective F1 hybrids. However, screening performed on individual plants of two parental 1ines and the corresponding hybrid indicated insufficient uniformity of parental lines, limiting the applicability of AFLP markers for testing hybrid seed purity.
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