Genetic mapping and localization of quantitative trait loci for chlorophyll content in Chinese cabbage (Brassica rapa ssp. pekinensis)
2012
Ge, Yu | Wang, Tao | Wang, Na | Wang, Zhe | Liang, Cui | Ramchiary, Nirala | Choi, Su-Ryun | Lim, Y. P. (Yong Pyo) | Piao, Zhong Yun
Chlorophyll a and chlorophyll b present in the chloroplasts of higher plants are the main pigments of leaf photosynthesis. The aim of this study was to localize the genetic factors affecting chlorophyll a and chlorophyll b content in the Chinese cabbage (Brassica rapa ssp. pekinensis) genome. To achieve this goal, we updated our previous genetic map, which was developed on the basis of an F₂ mapping population derived from a cross between 2 diverse Chinese cabbage lines, ‘501’ and ‘601’ with additional unigene-derived microsatellite markers. Eleven new polymorphic markers along with 227 previously mapped marker loci were used to construct a new updated B. rapa map containing 238 marker loci and covering a total length of 926.7cM, with an average distance of 3.9cM between markers. Quantitative trait loci (QTL) mapping using the chlorophyll a and chlorophyll b phenotypic data from an F₂:₃ mapping population over 2 years identified 10 QTL in 8 genetic intervals in the Chinese cabbage accounting for 7–17% of the observed phenotypic variation.
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