Effect of acid hydrolysis conditions on the bioactivity of isoflavone content in soybeans
2023
Lee, J.Y. | Kim, N.G. | Lee, Y.Y. | Kim, M.H. | Han, N.R. | Kang, M.S. | Kim, H.J.
This study investigated the effects of changes in soybean isoflavone and polyphenol content, under thermal and acid hydrolysis conditions, on antioxidant and α-glucosidase inhibitory activity. The contents of genistein, glycitein, and daidzein before the hydrolysis ranged from 10.53-167.68, 7.98-63.75, and 7.30-124.41 μg/mg extract, respectively, and increased upon acid hydrolysis. Total polyphenol content ranged from 32.34-52.59 μg GAE/mg, with the highest values observed at 1 h with 1M HCl. DPPH radical scavenging activity ranged from 8.13-14.70 TE/mg and further increased upon acid hydrolysis. Reducing power ranged from 49.12-217.56 μM FeSO₄/mg and was most effective when treated with 1M HCl for 8 h. The α-glucosidase inhibitory activity increased to 88.94% when treated with 1M HCl for 8 h, whereas control and thermal hydrolysis were ineffective. The results of this study can be used for the production of functional foods with enhanced bioactivity of soybeans.
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