Profiling of Major B6 Vitamers (Pyridoxine, Pyridoxal, and Pyridoxamine) in Fruits Commonly Consumed in Korea
2023
Lee, K.H. | Gwak, Y.J. | Chun, J.Y.
People consume fruit every day to prevent chronic diseases. However, contents of micronutrients such as vitamins involved in energy metabolism have not been determined for some fruits. In this study, we analyzed the B₆ vitamer (pyridoxine, pyridoxal, and pyridoxamine) contents of 22 commonly consumed fruits in Korea using high-performance liquid chromatography equipped with a fluorescence detector (HPLC-FLD). Vitamin B₆ levels ranged from 3.71∼198.32 μg/100 g, and passionfruit (198.32 μg/100 g) and apple mango (114.10 μg/100 g) had the highest values. Differences in the B6 vitamer contents were observed according to the degrees of ripeness and varieties of fruit, remarkably for plum, kiwi, and persimmon. HPLC-FLD showed excellent accuracy (100.0∼103.4% recoveries) and precision (repeatability <3% RSD and reproducibility 3.5∼6.7% RSD). Limits of detection and quantification showed that the technique used enabled analysis at low analyte levels. Results showed that simultaneous analysis of pyridoxine, pyridoxal, and pyridoxamine levels by HPLC-FLD provides a reliable means of determining vitamin B₆ values. Furthermore, the data obtained could be used to replace missing values for trace vitamin B6 levels to the National Standard Food Composition Table and as a guide for consumers.
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