CHLOROPHYLL DEGRADATION IN LEAVES OF NANOPARTICLES EXPOSED COTTON SEEDLINGS UNDER DARK CONDITION
2023
Ismat Ahmadov | Faride Hasanova
The aim of this study was to determine the effect of nanoparticles on the aging of cotton leaves by measuring the content of chlorophyll. Until now, the mechanism of chlorophyll degradation and the factors influencing this process have not been fully studied. In the presented research, the effect of nanoparticles on the degradation of chlorophyll and carotenoids, photosynthetic rate, Fv/Fm ratio and production of oxidants, ie. H2O2 and superoxide radical (O2-), lipoxygenase enzyme activity (LOX), and antioxidant enzymes SOD and CAT activity in 14-day-old cotton seedlings under dark conditions was investigated. Metal-based nanoparticles (ZnO, CuO, Fe2O3,TiO2) were used in the experiments. Cotton was grown in a controlled environment plant growth chamber. First, the compounds of chlorophyll and carotenoids were measured in the leaves of the third tiers of 14-day-old cotton seedlings, and then the seedlings were kept in the dark. The kinetics of the process depends on the composition and size of the nanoparticles. Nanoparticles do not significantly affect the increase of H2O2, superoxide anion during chlorophyll degradation, but ZnO nanoparticles do. Lipoxygenase, SOD and CAT enzymes activity in this process of nanoparticles is not seriously affected.
显示更多 [+] 显示较少 [-]