Sphinganine and sphingosine levels and ratio in urine and blood samples from a Catalonian population (Spain).
2011
Cano-Sancho, Germán | Marin, S. | Sanchis, V. | Colom, C. | Coronel, M.B. | Ramos, A.J. | Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA) ; Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)
International audience
显示更多 [+] 显示较少 [-]英语. Fumonisins occur mainly in maize, and produce alterations on sphingolipid metabolism, unbalancing sphinganine (Sa) / sphingosine (So) ratio. This alteration has been proposed as a biomarker of fumonisin exposure. The objective of the present work was to study the urinary and plasmatic levels of Sa, So as well as, the ratio Sa/So from a sample of Catalonian population exposed at low levels of fumonisins. Firstly, plasmatic and urinary Sa and So levels and the ratio Sa/So were compared between two population groups, and after, urinary Sa and So levels from corn-food consumers and a control group were monitored during two weeks under controlled intake of corn-foods. Sa and So levels were determined in urine and blood samples using validated methods using HPLC with fluorescence detection. Significant differences were not found in urine samples when Sa/So ratios were compared from corn-food consumers and non consumers, while significant differences were found in urine and plasma samples but evidences of mechanism of action of fumonisins were not apparent. Through time-course study, we have narrowed down the day in which the maximum alteration of Sa/So ratio should be expected in humans. In this paper we have reported some useful information to improve the design of studies to validate the ratio Sa/So as a possible biomarker of fumonisin exposure.
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