Characterization of Rosculus vilicus sp. nov., a rhizarian amoeba interacting with Mycobacterium avium subsp. paratuberculosis
2023
Jessu, Amélie | Delafont, Vincent | Moyen, Jean-Louis | Biet, Franck | Samba-Louaka, Ascel | Héchard, Yann | Écologie et biologie des interactions [UMR 7267] (EBI [Poitiers]) ; Université de Poitiers = University of Poitiers (UP)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire Départemental d'Analyse et de Recherche de la Dordogne (LDAR24) | Infectiologie et Santé Publique (ISP) ; Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | ANR-22-CE35-0010,Amitub,Les amibes environnementales, réservoir et vecteur de la tuberculose et de la paratuberculose bovines ?(2022)
International audience
显示更多 [+] 显示较少 [-]英语. Free-living amoebae are described as potential reservoirs for pathogenic bacteria in the environment. It has been hypothesized that this might be the case for Mycobacterium avium subsp. paratuberculosis, the bacterium responsible for paratuberculosis. In a previous work, we isolated an amoeba from a water sample in the environment of infected cattle and showed that this amoeba was associated with Mycobacterium avium subsp. paratuberculosis. While a partial 18S rRNA gene has allowed us to suggest that this amoeba was Rosculus-like, at that time we were not able to sub-cultivate it. In the present study, we succeeded in cultivating this strain at 20–25°C. This amoeba is among the smallest (5–7 μm) described. The sequencing of the whole genome allowed us to extract the full 18S rRNA gene and propose this strain as a new species of the Rosculus genus, i.e., R. vilicus. Of note, the mitochondrial genome is particularly large (184,954 bp). Finally, we showed that this amoeba was able to phagocyte Mycobacterium avium subsp. paratuberculosis and that the bacterium was still observed within amoebae after at least 3 days. In conclusion, we characterized a new environmental amoeba species at the cellular and genome level that was able to interact with Mycobacterium avium subsp. paratuberculosis. As a result, R. vilicus is a potential candidate as environmental reservoir for Mycobacterium avium subsp. paratuberculosis but further experiments are needed to test this hypothesis.
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