Identification of sympatric cryptic species of Aedes albopictus subgroup in Vietnam: new perspectives in phylosymbiosis of insect vector
2017
Minard, Guillaume | Tran Van, Van | Tran, Florence Hélène | Melaun, Christian | Klimpel, Sven | Koch, Lisa Katharina | Ly Huynh Kim, Khanh | Huynh Thi Thuy, Trang | Tran Ngoc, Huu | Potier, Patrick | Mavingui, Patrick | Valiente Moro, Claire | Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon | Centre National de la Recherche Scientifique (CNRS) | Institut National de la Recherche Agronomique (INRA) | Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM) ; Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS) | Université de Lyon | Senckenberg Biodiversity and Climate Research Centre (SBiK-F) ; Goethe University Frankfurt = Goethe-Universität Frankfurt am Main-Senckenberg – Leibniz Institution for Biodiversity and Earth System Research - Senckenberg Gesellschaft für Naturforschung ; Leibniz Association-Leibniz Association | Institute of Ecology, Evolution and Diversity ; Goethe University Frankfurt = Goethe-Universität Frankfurt am Main | Institut Pasteur d'Ho Chi Minh Ville ; Pasteur Network (Réseau International des Instituts Pasteur) | Processus Infectieux en Milieu Insulaire Tropical (PIMIT) ; Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS) | EC2CO CNRS; CMIRA Region Rhone-Alpes; ERA-NET BiodivERsA ANR-13-EBID-0007-01 FWF I-1437; DFG KL 2087/6-1; FR41 Bio-Environment and Health (University Lyon 1);Academy of Finland 273098 265641 | ANR-13-EBID-0007,GC-INVAMOFECT,GLOBAL CHANGE AND INVASIVE MOSQUITOES AS INFECTIOUS DISEASE RISKS IN EUROPE(2013)
International audience
显示更多 [+] 显示较少 [-]英语. Background: The Aedes (Stegomyia) albopictus subgroup includes 11 cryptic species of which Ae. albopictus is the most widely distributed. Its global expansion associated with a documented vector competence for several emerging arboviruses raise obvious concerns in the recently colonized regions. While several studies have provided important insights regarding medical importance of Ae. albopicus, the investigations of the other sibling species are scarce. In Asia, indigenous populations within the Ae. albopictus subgroup can be found in sympatry. In the present study, we aimed to describe and compare molecular, morphological and bacterial symbionts composition among sympatric individuals from the Ae. albopictus subgroup inhabiting a Vietnamese protected area.Results: Based on morphological structure of the cibarial armarture, we identified a cryptic species in the forest park at Bù Gia Mập in the south-eastern region of Vietnam. Analysis of nuclear (ITS1-5.8S-ITS2) and mitochondrial (cox1, nad5) markers confirmed the divergence between the cryptic species and Ae. albopictus. Analysis of midgut bacterial microbiota revealed a strong similarity among the two species with a notable difference; contrary to Ae. albopictus, the cryptic species did not harbour any Wolbachia infection.Conclusions: These results could reflect either a recent invasion of Wolbachia in Ae. albopictus or alternatively a loss of this symbiont in the cryptic species. We argue that neglected species of the Ae. albopictus subgroup are of main importance in order to estimate variation of host-symbionts interactions across evolution.
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