Survivability of boar sperm diluted in long-term extender containing kofola
2021
Frydrychova, Sona | Lustykova, Alena | Seifert, Josef et al.
The objective of this study was to evaluate the survivability of boar sperm diluted in long-term extender containing kofola. Eight sperm rich fractions from 4 fertile boars from one AI centre with motility ≥80% and the number of morphologically abnormal spermatozoa ≤25% were used in this study. Kofola original – KO was added to boar semen extender Androstar plus (AS+). Semen was diluted in AS+ as a control (K) and AS+ with KO in the dilution ratio 1+2. Samples were stored at 17°C and evaluated after 0h, 48h, 92h and 168h storage time for sperm motility and sperm viability. SCA system was used for determined sperm motility (total, progressive, non-progressive and immotile) and kinetic characteristics - straight line velocity (VSL, μm/s), average path velocity (VAP, μm/s), curvilinear velocity (VCL, μm/s), straightness (STR, VSL/VAP, %), linearity (LIN, VSL/VCL, %), wobble (WOB, VAP/VCL, %). Statistically significant differences (P<0.05) were recorded between K (94.47, 73.91, 5.53%) vs. KO (87.76, 61.10, 12.24%) in total mean values of total sperm motility, progressive and immotile sperm. The progressive sperm motility was 13% lower in KO than K at 96h storage time then showed a sharp decrease after 168 h. The sperm survival was significantly affected by storage time (P<0.05). Between kinetic indicator of speed not reported significant differences between K vs. KO (P>0.05). No difference between K (77.75%) vs. KO (79.44) were found in percentage of sperm viability (P>0.05). In conclusion, the results of motility and viability point to the possibility of using kofola original as a potential ingredient in longterm boar extender until the storage time of 96 hours.
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