Associative effects between red clover and Kikuyu grass silage: Proteolysis reduction and synergy during in vitro organic matter degradation
2017
Cristina Guzattia, Gabriela | Gonçalves Duchini, Paulo | Vilmar Kozloskib, Gilberto | Niderkorn, Vincent | Mendonça Nunes Ribeiro-Filhoa, Henrique | Departamento de Produção Animal e Alimentos ; Universidade do Estado de Santa Catarina | Departamento de Zootecnia ; Universidade Estadual de Maringá [Brasil] = State University of Maringá [Brazil] = Université d'État de Maringá [Brésil] (UEM) | Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH) ; Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS) | CAPES/FAPESC (Brazil); CNPq (Brazil); FAPESC/UDESC PAP - 01/2016; CAPES/PROAP
The aim of this study was to evaluate the effects of the association between red clover (RC;Trifolium pratense) and a tropical grass (kikuyu grass) on the proteolysis of ensiled material andthe in vitro degradation of protein and organic matter. Red clover and kikuyu grass were ensiledin the following proportions: 0:1000, 250:750, 500:500, 750:250, and 1000:0 g/kg of dry matter(DM). The fraction of rapidly degradable protein, the ammonia nitrogen (NH3-N) content of thesilo, the in vitro protein degradation, and the degradation rate decreased linearly (P < 0.001) asthe RC content in the ensiled material increased. Cumulative gas production after 24 h incubationshowed a positive quadratic effect when RC was increased to 500 g/kg (P < 0.001). The silageswith the highest RC content reduced proteolysis more effectively during ensiling and ruminalfermentation. Inter-species synergistic effects positively affected in vitro gas production, whichwas optimal when RC and kikuyu grass were ensiled in the same proportions as that of total DM.
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