Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L.
2008
Dubreuil, Marta | Sebastiani, Federico | Mayol, Maria | González-Martínez, Santiago C. | Riba, Miquel | Vendramin, Giovanni G. | Centre for Ecological Research and Applied Forestries = Centre de Recerca Ecologica i Aplicacions Forestals (CREAF) ; Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain] (CSIC) | Università degli Studi di Firenze = University of Florence = Université de Florence (UniFI) | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria = National Institute for Agricultural and Food Research and Technology (INIA) | National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR)
International audience
显示更多 [+] 显示较少 [-]英语. Seven polymorphic nuclear microsatellite markers for Taxus baccata L. (English yew) were developed using an enriched-library method. An additional polymorphic SSR was obtained by testing eight primer pairs from the congeneric species Taxus sumatrana. Mendelian inheritance for the seven Taxus baccata SSRs was proved by genotyping 17 individuals and 124 megagametophytes (conifer seed haploid tissue). A total of 96 individuals from 5 different populations (10–26 samples per population) were used to estimate genetic diversity parameters. High levels of genetic diversity, with values ranging from 0.533 to 0.929 (6–28 alleles per SSR) were found. No linkage disequilibrium between pairs of loci was detected. All loci but one showed significant departures from Hardy–Weinberg equilibrium. Excess of homozygosity was probably due to high inbreeding in English yew populations, an outcome of low effective population size and/or fragmented distribution. Highly polymorphic SSRs will be used to conduct population genetic studies at different geographical scales and to monitor gene flow.
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