Study of cloudberry Rubus chamaemorus L. genetic diversity in Latvia and Belarus based on different molecular marker systems
2021
Krasņevska, Nikole | Butkauskas, Dalius | Samokhvalova, Natalia | Miķelsone, Andra | Kruchonok, Alesia | Grauda, Dace
Cloudberry (Rubus chamaemorus L.) is a perennial plant species from the genus Rosaceae with subarctic and boreal circumpolar distribution mainly in the Northern Hemisphere. In Europe, R. chamaemorus is widespread in Fennoscandia and Baltic countries (Thiem, 2003). In Central European countries R. chamaemorus occurs in small, isolated populations, and can be observed as a glacial relict (Ehrich et al., 2008). In Belarus, R. chamaemorus occurs only in the northern part of the country, and it is considered a critically endangered species with high protection level (Kachanovsky, 2015). In this study, iPBS (inter-primer binding site) retrotransposon-based molecular markers marker system described by Kalendar et al. (2010) in combination with RChL-Ch1 and RChR-Ch1 primers designed for amplification and sequencing 5.8S ribosomal RNA coding fragment was used to assess the genetic diversity and population genetic structure of Latvian and Belarus natural populations of R. chamaemorus. In total 276 samples from 12 populations, eight from Latvia and four from Belarus were analysed mainly by comparison iPBS polymorphism results.
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出版者 The University of Latvia Press