Optimum dose of gamma irradiation to inactivate Vibrio paraheamolyticus in fresh and freeze-dried
2015
Heidarieh, Marziye | Soltani, Mahdi | Motamedi sedeh, Farahnaz | Rajabifar, Saeed | Ahmadi, Mohammad Reza | Shahhoseini, Gholamreza
BACKGROUND: Vibrio paraheamoloyticus is one the causative agents of vibriosis with high mortality in farmed fish and shrimp and under predisposing conditions. OBJECTIVES: This study was aimed to assess the effect of gamma irradiation on the inactivation of V. paraheamoloyticus under fresh and freeze-dried conditions. METHODS: Vials of 0.5 ml fresh culture of the bacterial suspensions at 1.5*1010 cfu/ml were subjected to 1, 1.5,2, 2.5, 3 and 4 KGy. Also, vials of 0.5 ml freeze-dried culture of the bacterial suspensions at 1.5 x 1010 cfu/ml were subjected to 2, 4, 7 and 8 KGy. The bacterial growth behavior was then evaluated on fresh medium. RESULTS: The obtained results showed that the minimum doses of 4 and 10 KGy were sufficient for the inactivation of fresh and freeze-dried bacteria, respectively. CONCLUSIONS: The result of this study shows that inactivation of V. paraheamolyicus in fresh culture condition requires below half- dosage of gamma ray required for the inactivation of the freeze- dried of bacterial cells.
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