Effect of different salinity concentration on gill of benni Barbus sharpeyi
2017
Morovvati, Hassan | Abdi, Rahim | Shamsi, Mohammad Mahdi
BACKGROUND: Benni of cyprinidae family is important for nutrition and economic and commercial species in the south of Iran and the Persian Gulf region. OBJECTVES: To investigate the importance of compatibility of fishes with different salinity in different areas and ability of osmoregulation along with maintenance of homeostasis. METHODS: For this study, 144 healthy Barbus sharpeyi with an average weight of 350±2.36 grams and length 25±1.25 cm in five groups were studied. The first group as control was located in municipal dechlorinated water and the next four groups respectively were kept in salinity 4ppt, 8ppt, 12 ppt and 16ppt in the same condition. On days 1, 3, 7, 14, 21 and 28 the second gill arch with maximum thickness of 0.5 cm from the left were prepared and placed in Bouin’s solution.Then the standard method of paraffin sections was done and tissue sections, 5-6 micrometer thick were prepared and stained with H&E methods. RESULT: Results showed the gradual transfer of fish to water with high salinity caused obvious changes in the number and distribution of chloride cells on different days. In particular, the changes in the size of these cells in two positions, filament and Lamella were evident. CONCLUSIONS: Due to the decrease of freshwater resources, from this research it can be concluded that the Barbus sharpeyi can resist the salinity without tissue changing. These findings suggest that Barbus sharpeyi is compatible with salinity and the concentration of 4ppt was optimum and concentrations of 8ppt and 12 ppt were tolerable.
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