Study of genetic structure of Rutilus frisii kutum in Golestan province coastal waters using microsatellite markers
2010
Rezaei, M. | Shabani, A. | Shabanpour, B. | Kashiri, H.
To determine the genetic diversity of Rutilus frisii kutum, 25 samples were collected from Gharesou and Gorgan-rud rivers in April 2008. The genomic DNA was extracted from the caudal fin using phenol- chloroform method and investigated with 10 microsatellite loci. All the 10 investigated loci were polymorphic and showed variability in the two populations of the rivers. The mean number of allele per locus at population level and the observed heterozygosity ranged from 4 to 13 and 0.22 to 1.00, respectively. The FST value between populations was 0.014 (P<0.05), and the analysis of genetic variation distribution indicated a very high within population variation (99%) while among population variation is low (1%). Highly significant deviation from hardy-Weinberg equilibrium was observed in both populations which can be attributed either to the presence of null alleles or to the genetic bottleneck. The results indicated a high level of genetic variation in this species and we concluded that there is more than one population of Rutilus frisii kutum in Golestan coastal waters.
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