Detection and identification of carriers of White spot Virus in wild shrimps and crabs in Persian gulf and Oman Sea(Hormozgan province)
2009
Gharavy, Behrooz | Afsharnasab, M. | Tmadoni, S. | Kamali, E. | Sadeghi, M.R. | Kakoolaki, S. | Karimzadeh, R. | Behzadi, S. | Karimi, A.
More than 20 viruses have been reported as pathogenic to shrimp.WSV has been found to be highly pathogenic not only to penaeid shrimps, but also to a wide range of hosts which include marine crabs , copepods, freshwater crabs and prawns. Main objective of this study was detection and identification of white spot virus from wild shrimp and crabs population on the coastal waters of Hormozgan Province.The samples were collected from three area seasonally include: coastal waters of Qeshm Island, Hengam Island and Jask. In this survey have been examined 1080 shrimp from each species of P.indicus, P.semisulcatus, P.merguiensis, Metapenaeus affinis and 1080 crabs (gill organs) by PCR and histopathological methods. Diagnostic kit for this survey have been prepared from Genesis Biotechnology CO.in Malaysia, so called "Single-Tube Nested PCR for WSSV". The analysis results revealed that all samples which examined from these area were free from WSV. Following PCR tests that were negative for all samples so no observed any damages of histology due to WSV on gills.
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