Screening for WSSV, IPNV and YHV in Astacus leptodactylus on water resources of Northwest Iran
2016
Nekuie Fard, Ali | Sharifrohani, M. | Zorriehzahra, J. | Afsharnasab, M. | Abdi, K. | Kakoolaki, S. | Seidgar, M. | Ghajari, A. | Azadikhah, D. | Davarrad, M. | Hanaei, M. | Mostafazadeh, B. | Taheri, M.M. | Shirvalilo, M. | Shiri, S. | Ganji, S. | Ahmadi, H. | Simroni, M.M. | Seyyedi, A. | Norozi, M.
Early recognition of diseases and pathogens transmitted from freshwater crayfish provides the possibility of early intervention and management in this regard and decreases the mortality and morbidity from infectious diseases. Hence, The awareness from presence and incidence rates of WSSD, YHD, IPN viruses are very important due to they are the most important zoonotic and infectious viruses in aquatic animals and crustaceans. Also they cause serious reduction of the amount and quality of freshwater crayfish in the world. For this region, this study was carried out for the first time to detect the mentioned viruses in freshwater crayfish from North West of Iran. In this study, a total of 725 crayfish (145 specimens from each water resource) were sampled during 2013 -2014 from dam reserviors including : Aras ( West Azarbaijan, Caspian Sea Basin),Ardalan ( East Azarbaijan, Urmia lake basin), Kazarj and karaghyeh (East Azarbaijan, Caspian Sea Basin),Shorabil(Ardabil, Caspian Sea Basin).All specimens were transferred to aquatic animals health and genetics laboratory of Iranian Artemia Research Center as live individuals under controlled condition. In order to virus isolation, the gill, Hepatopancreas, Hemolymph and pleopods were tested using PCR and RT-PCR methods. Suspicious samples were sent to the reference viral diseases diagnosis laboratory of Iranian veterinary organization. Screening results showed the probability of no contamination in freshwater crayfish from studied water resources. Therefore, it is recommended continuation of screening mentioned viruses by the authorities placed on the agenda.
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