Assessment of Aspergillus Fumigatus Burden in Lungs of Intratracheally-Challenged Turkeys (Meleagris Gallopavo) by Quantitative PCR, Galactomannan Enzyme Immunoassay, and Quantitative Culture
2014
Melloul, Elise | Thierry, Simon | Durand, Benoit | Cordonnier-Lefort, Nathalie | Desoubeaux, Guillaume | Chandenier, Jacques | Bostvironnois, Christophe | Botterel, Françoise | Chermette, René | Guillot, Jacques | Arné, Pascal | Dynamic Microbiology - EA 7380 (DYNAMIC) ; École nationale vétérinaire d'Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est (UPE)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR) ; École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) | Laboratoire d'études et de recherches en pathologie animale et zoonoses ; Agence Française de Sécurité Sanitaire des Aliments (AFSSA) | Ecole Nationale Vétérinaire d’Alfort, Unité d’anatomie pathologique ; Université Paris-Est (UPE) | Centre Hospitalier Régional Universitaire de Tours (CHRU Tours) | Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR) ; Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM) | Université de Tours (UT)
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显示更多 [+] 显示较少 [-]英语. Assessment of Aspergillus fumigatus burden in lungs of intratracheally-challenged turkeys (Meleagris gallopavo) by quantitative PCR, galactomannan enzyme immunoassay, and quantitative culture. Comp Immunol Microbiol Infect Dis (2014), http://dx. a b s t r a c t Aspergillus fumigatus remains a major respiratory pathogen in birds and treatment is still difficult. We challenged different groups of few-day-old turkeys via intratracheal aerosoli-sation with increasing concentrations (10 5 up to 10 8) of conidia using a MicroSprayer ® device. The fungal burden was assessed by real-time PCR, galactomannan dosage, CFU counting and histopathological evaluation in order to provide a comparison of these results within each inoculum groups. Significant mortality, occurring in the first 96 h after inoculation , was only observed at the highest inoculum dose. Culture counts, GM index and qPCR results on the one hand and inoculum size on the other hand appeared to be clearly correlated. The mean fungal burden detected by qPCR was 1.3 log 10 units higher than the mean values obtained by CFU measurement. The new model and the markers will be used to evaluate the efficacy of antifungal treatments that could be used in poultry farms.
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