Regulation of FUSCA3 Expression During Seed Development in Arabidopsis
2019
Roscoe, Thomas James | Vaissayre, Virginie | Paszkiewicz, Gaël | Clavijo, Fernando | Kelemen, Zsolt | Michaud, Caroline | Lepiniec, Loic | Dubreucq, Bertrand, B. | Zhou, Dao-Xiu | Devic, Martine | Diversité, adaptation, développement des plantes (UMR DIADE) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [Occitanie]) | Eberhard Karls Universität Tübingen = University of Tübingen | Laboratoire de chimie bactérienne (LCB) ; Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) | Institut Jean-Pierre Bourgin (IJPB) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)) ; Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire d'Océanographie Microbienne (LOMIC) ; Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB) ; Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) | the French NationalResearch Agency [ANR; grant CERES (ANR-10-BLAN-123801)and ANR-BLAN-10-123801 to V.V., F.C. and G.P.]. The InstitutJean-Pierre Bourgin benefits from the support of the LabexSaclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS] | ANR-10-BLAN-1238,CERES,Contrôle de l'expression des gènes du REseau de régulation "AFL" et développement de la graine(2010) | ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010)
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显示更多 [+] 显示较少 [-]英语. FUSCA3 (FUS3) is a master regulator of seed development important in establishing and maintaining embryonic identity whose expression is tightly regulated at genetic and epigenetic levels. Despite this prominent role, the control of FUS3 expression remains poorly understood. Promoter and functional complementation analyses provided insight into the regulation of FUS3. W-boxes present in the promoter proximal to the start of transcription are recognized by WRKY type-1 factors which are necessary for the activation of FUS3 expression. The RY motif, the binding site of B3 factors, is important for the activation of FUS3 in the embryo proper but not in the suspensor. The loss of a negative regulatory sequence (NRS) leads to preferential expression of FUS3 in the vasculature of vegetative tissues. Since the NRS includes the RY motif, mechanisms of activation and repression target adjacent or overlapping regions. These findings discriminate the regulation of FUS3 from that of LEAFY COTYLEDON2 by the control exerted by WRKY factors and by the presence of the RY motif, yet also confirm conservation of certain regulatory elements, thereby implicating potential regulation by BASIC PENTACYSTEINE (BPC) factors and POLYCOMB REPRESSIVE COMPLEX2 (PRC2).
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