In vitro maturation affects chromosome segregation, spindle morphology and acetylation of lysine 16 on histone H4 in horse oocytes
2017
Franciosi, Federica | Goudet-Guitton, Ghylène | Tessaro, Irene | Papillier, Pascal | Dalbies-Tran, Rozenn | Reigner, Fabrice | Deleuze, Stefan | Douet, Cécile | Miclea, Ileana | Lodde, Valentina | Luciano, Alberto M. | Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety ; Università degli Studi di Milano = University of Milan (UNIMI) | Physiologie de la reproduction et des comportements [Nouzilly] (PRC) ; Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) | Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO) ; Institut National de la Recherche Agronomique (INRA) | Clinique des Animaux de Compagnie et des Équidés ; Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège) | University of Agricultural Sciences and Veterinary Medicine | Regione Sardegna and Regione Lombardia, project "Ex Ovo Omnia" (Grant no. 26096200 to A.M.L.); L’Oreal Italia per le Donne e la Scienza 2012 fellowship (Contract 2012 to F. F.), by FP7-PEOPLE-2011-CIG, Research Executive Agency (REA) ‘Pro-Ovum’ (Grant no. 303640 to V.L.); European Social Fund, Sectorial Operational Program for Human Resource Development 2007–2013 (Contract no. POSDRU/89/1.5/S/62371 to I.M.)
Implantation failure and genetic developmental disabilities in mammals are caused by errors in chromosome segregation originating mainly in the oocyte during meiosis I. Some conditions, like maternal ageing or in vitro maturation (IVM), increase the incidence of oocyte aneuploidy. Here oocytes from adult mares were used to investigate oocyte maturation in a monovulatory species. Experiments were conducted to compare: (1) the incidence of aneuploidy, (2) the morphology of the spindle, (3) the acetylation of lysine 16 on histone H4 (H4K16) and (4) the relative amount of histone acetyltransferase 1 (HAT1), K(lysine) acetyltransferase 8 (KAT8, also known as MYST1), histone deacetylase 1 (HDAC1) and NAD-dependent protein deacetylase sirtuin 1 (SIRT1) mRNA in metaphase II stage oocytes that were in vitro matured or collected from peri-ovulatory follicles. The frequency of aneuploidy and anomalies in spindle morphology was increased following IVM, along with a decrease in H4K16 acetylation that was in agreement with our previous observations. However, differences in the amount of the transcripts investigated were not detected. These results suggest that the degradation of transcripts encoding for histone deacetylases and acetyltransferases is not involved in the changes of H4K16 acetylation observed following IVM, while translational or post-translational mechanisms might have a role. Our study also suggests that epigenetic instabilities introduced by IVM may affect the oocyte and embryo genetic stability.
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