Reduction in SARS-CoV-2 Virus Infectivity in Human and Hamster Feces
2022
Wurtzer, Sébastien | Lacote, Sandra | Murri, Severine | Marianneau, Philippe | Monchatre-Leroy, Elodie | Boni, Mickaël | Ferraris, Olivier | Maday, Yvon | Kébé, Ousmane | Dia, Ndongo | Peyrefitte, Christophe | Sokol, Harry | Moulin, Laurent | Maréchal, Vincent | Eau de Paris | Laboratoire de Lyon [ANSES] ; Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | Mycoplasmoses animales (MYCO) ; Laboratoire de Lyon [ANSES] ; Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS) | Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN) ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) | Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA) | Laboratoire Jacques-Louis Lions (LJLL (UMR_7598)) ; Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité) | Institut universitaire de France (IUF) ; Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.) | Institut Pasteur de Dakar ; Pasteur Network (Réseau International des Instituts Pasteur) | Institut Pasteur de la Guyane ; Pasteur Network (Réseau International des Instituts Pasteur) | Centre de Recherche Saint-Antoine (CRSA) ; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU) | FHU Paris Center for Microbiome Medicine (FHU PaCeMM) ; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU) | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Analyses were supported by the OBEPINE Research grant (French ministry of Research), as well as the specific financial participation of Eau de Paris, IRBA, and the Institut Pasteur Dakar. | Obépine Consortium
International audience
显示更多 [+] 显示较少 [-]英语. Objective: There is extensive evidence that SARS-CoV-2 replicates in the gastrointestinal tract. However, the infectivity of virions in feces is poorly documented. Although the primary mode of transmission is airborne, the risk of transmission from contaminated feces remains to be assessed.Design: The persistence of SARS-CoV-2 (infectivity and RNA) in human and animal feces was evaluated by virus isolation on cell culture and RT-qPCR, respectively. The exposure of golden Syrian hamsters to experimentally contaminated feces through intranasal inoculation has also been tested to assess the fecal-oral transmission route.Results: For periods that are compatible with average intestinal transit, the SARS-CoV-2 genome was noticeably stable in human and animal feces, contrary to the virus infectivity that was reduced in a time- and temperature-dependent manner. In human stools, this reduction was variable depending on the donors. Viral RNA was excreted in the feces of infected hamsters, but exposure of naïve hamsters to feces of infected animals did not lead to any productive infection. Conversely, hamsters could be experimentally infected following exposure to spiked fresh feces.Conclusion: Infection following exposure to naturally contaminated feces has been suspected but has not been established so far. The present work demonstrates that SARS-CoV-2 rapidly lost infectivity in spiked or naturally infected feces. Although the possibility of persistent viral particles in human or animal feces cannot be fully ruled out, SARS-CoV-2 transmission after exposure to contaminated feces is unlikely.
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