PCR based differentiation of pathogenic and non pathogenic Leptospira using hap1 gene
2010
KUMAR, S SENTHIL | PARTHIBAN, M | MAGUDESWARAN, S K | KUMANAN, K
A polymerase chain reaction (PCR) was developed for diagnosing leptospirosis as well as for differentiating pathogenic and saprophytic leptospires. The integral hap1 gene was present only in the pathogenic Leptospira strains. Hence, PCR amplification was done using both pathogenic and non pathogenic leptospira using hap1 gene specific primers. The expected amplicon size of 262 bp was obtained for all pathogenic strains tested. On the other hand, no amplification was obtained in saprophytic leptospira strains used.
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书目信息
出版者
Indian Council of Agricultural Research
其它主题
Saprophytic leptospira; Hap1 gene
语言
英语
格式
application/pdf
许可
Copyright (c) 2014 The Indian Journal of Animal Sciences
类型
Journal Article; Journal Part
来源
The Indian Journal of Animal Sciences; Vol. 80 No. 5 (2010), 2394-3327, 0367-8318
2024-07-23
2025-10-25
Dublin Core