Molecular studies on protein- and carbohydrate-converting ezymes from thermophilic bacteria
2004
Kluskens, L.D.
Microorganisms that are able to grow at hightemperatures are calledthermophiles(>55°C) orhyperthermophiles(>80°C). Growth at high temperatures involvesthermostableenzymes, and the question how this stabilization takes place at a structural level has played a central role in research of these organisms. In addition, the interest in applying them inindustry as biocatalysts has largely increased.The bacterial domain holds a few (hyper)thermophilicmembers, and the organisms that were used in this study, Fervidobacterium pennivorans , F. gondwanense and Thermotogamaritima , belong to theThermotogalesorder. They grow optimally from 65 to 80°C and are able to use a large variety of carbohydrates and peptides, for which they possess a range of enzymes. This research describes the detailed molecular and biochemical characterization of enzymes that enable growth of these organisms on, occasionally, remarkable substrates like pectin or chicken feathers. The study on these so-called thermozymes is an example of an integrated approach in which bioinformatics, molecular genetics, structural analysis, DNA microarray analysis, biochemistry and fermentation has been combined.
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