The genome of Nectria haematococca: contribution of supernumerary chromosomes to gene expansion
2009
Coleman, Jeffrey J. | Rounsley, Steve D. | Rodriguez-Carres, Marianela | Kuo, Alan | Wasmann, Catherine C. | Grimwood, Jane | Schmutz, Jeremy | Taga, Masatoki | White, Gerard J. | Zhou, Shiguo | Schwartz, David C. | Freitag, Michael | Ma, Li-Jun | Danchin, Etienne | Henrissat, Bernard | Coutinho, Pedro, M | Nelson, David R. | Straney, Dave | Napoli, Carolyn A. | Barker, Bridget M. | Gribskov, Michael | Rep, Martijn | Kroken, Scott | Molnár, István | Rensing, Christopher | Kennell, John C. | Zamora, Jorge | Farman, Mark L. | Selker, Eric U. | Salamov, Asaf | Shapiro, Harris | Pangilinan, Jasmyn | Lindquist, Erika | Lamers, Casey | Grigoriev, Igor V. | Geiser, David M. | Covert, Sarah F. | Temporini, Esteban | Vanetten, Hans D. | University of Arizona | Massachusetts General Hospital [Boston] | Department of Biology ; Duke University [Durham] | Joint Genome Institute (JGI) | HudsonAlpha Genome Sequencing Center ; University of Alabama [Huntsville] (UAH) | Okayama University | University of Wisconsin-Madison | Department of biochemistry and biophysics ; Oregon State University (OSU) | Broad Institute of MIT and Harvard (BROAD INSTITUTE) ; Harvard Medical School [Boston] (HMS)-Massachusetts Institute of Technology (MIT)-Massachusetts General Hospital [Boston] | Interactions Biotiques et Santé Végétale (IBSV) ; Institut National de la Recherche Agronomique (INRA) | Architecture et fonction des macromolécules biologiques (AFMB) ; Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) | Université de la Méditerranée - Aix-Marseille 2 | Department of Molecular Sciences ; The University of Tennessee Health Science Center [Memphis] (UTHSC) | Department of Cell Biology and Molecular Genetics | Department of Biological Sciences [Lafayette IN] ; Purdue University [West Lafayette] | Vrije Universiteit Amsterdam [Amsterdam] (VU) | Department of Soil, Water and Environmental Science ; University of Arizona | Department of Biology ; University of Memphis | Department of Plant Pathology ; University of Kentucky (UK) | Institute of Molecular Biology ; University of Oregon [Eugene] | Pennsylvania State University (Penn State) ; Penn State System | Warnell School of Forestry and Natural Resources ; University of Georgia [USA]
International audience
显示更多 [+] 显示较少 [-]英语. The ascomycetous fungus Nectria haematococca, (asexual name Fusarium solani), is a member of a group of >50 species known as the "Fusarium solani species complex". Members of this complex have diverse biological properties including the ability to cause disease on >100 genera of plants and opportunistic infections in humans. The current research analyzed the most extensively studied member of this complex, N. haematococca mating population VI (MPVI). Several genes controlling the ability of individual isolates of this species to colonize specific habitats are located on supernumerary chromosomes. Optical mapping revealed that the sequenced isolate has 17 chromosomes ranging from 530 kb to 6.52 Mb and that the physical size of the genome, 54.43 Mb, and the number of predicted genes, 15,707, are among the largest reported for ascomycetes. Two classes of genes have contributed to gene expansion: specific genes that are not found in other fungi including its closest sequenced relative, Fusarium graminearum; and genes that commonly occur as single copies in other fungi but are present as multiple copies in N. haematococca MPVI. Some of these additional genes appear to have resulted from gene duplication events, while others may have been acquired through horizontal gene transfer. The supernumerary nature of three chromosomes, 14, 15, and 17, was confirmed by their absence in pulsed field gel electrophoresis experiments of some isolates and by demonstrating that these isolates lacked chromosome-specific sequences found on the ends of these chromosomes. These supernumerary chromosomes contain more repeat sequences, are enriched in unique and duplicated genes, and have a lower G+C content in comparison to the other chromosomes. Although the origin(s) of the extra genes and the supernumerary chromosomes is not known, the gene expansion and its large genome size are consistent with this species' diverse range of habitats. Furthermore, the presence of unique genes on supernumerary chromosomes might account for individual isolates having different environmental niches
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