Similarities in Leptospira serogroup and species distribution in animals and humans in the Indian ocean island of Mayotte
2012
Desvars, Amélie | Naze, Florence | Vourc’h, Gwenaël | Cardinale, Eric | Picardeau, Mathieu | Michault, Alain | Bourhy, Pascale | Laboratoire de Bactériologie-Parasitologie-Virologie-Hygiène ; Groupe Hospitalier Sud Réunion (GHSR) | Unité de Recherche d'Épidémiologie Animale (UR EpiA) ; Institut National de la Recherche Agronomique (INRA) | Centre de Recherche et de Veille sur les Maladies Émergentes dans l'Océan Indien (CRVOI) ; Université de La Réunion (UR) | Biologie des Spirochètes / Biology of Spirochetes ; Institut Pasteur [Paris] (IP) | This work was funded by the Institut Pasteur and the French Ministry of Health (InVS)
International audience
显示更多 [+] 显示较少 [-]英语. Our objective was to identify local animal reservoirs of leptospirosis to explain the unusual features of Leptospira strains recently described among patients on the island of Mayotte. By means of a microscopic agglutination test using local clinical isolates, we found that 11.2% of black rats were seropositive to Leptospira, whereas 10.2% of flying foxes, 2% of lemurs, 93.1% of domestic dogs, and 87.5% of stray dogs were seropositive. As observed in humans, Mini was the main serogroup circulating in animals, whereas serogroup Icterohaemorrhagiae was absent. Using quantitative polymerase chain reaction, we also showed that 29.8% of rats carried leptospires in their kidneys. The sequencing of 16S rRNA gene sequences of Leptospira found in black rat kidneys identified four genomospecies (Leptospira borgpetersenii, Leptospira interrogans, Leptospira kirschneri, and L. borgpetersenii group B), which established black rats as the major source of leptospirosis transmission to humans. The origins of such a genetic diversity in Leptospira strains are discussed.
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