Assessment of the Probiotic Potential of a Dairy Product Fermented by [i]Propionibacterium freudenreichii[/i] in Piglets
2012
Cousin, Fabien | Foligné, Benoît | Deutsch, Stéphanie-Marie | Massart, Sébastien | Parayre-Breton, Sandrine | Le Loir, Yves | Boudry, Gaëlle | Jan, Gwénaël | Science et Technologie du Lait et de l'Oeuf (STLO) ; Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Centre National Interprofessionnel de l'Economie Laitière [Paris] (CNIEL) | Lactic Acid Bacteria & Mucosal Immunity - CIIL ; Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL) ; Institut Pasteur de Lille ; Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [CHU Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille ; Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [CHU Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS) | Université Lille Nord de France (COMUE) | DNA Vision SA | Alimentation Adaptations Digestives, Nerveuse et Comportementales (ADNC) ; Institut National de la Recherche Agronomique (INRA) | Conseil Régional de Breatgne, CRITT santé (Prodico), CBB Développement ( profusion), CNIEL/Syndifrais, ANR -10-ALIA-016
Dairy propionibacteria, including Propionibacterium freudenreichii, display promising probiotic properties, including immunomodulation. These properties are highly strain-dependent and rarely studied in a fermented dairy product. We screened 10 strains, grown in a newly developed fermented milk ultrafiltrate, for immunomodulatory properties in vitro. The most anti-inflammatory strain, P. freudenreichii BIA129, was further tested on piglets. P. freudenreichii-fermented productimproved food intake and growth of piglets. Colonic mucosa explants of treated pigs secreted less interleukin 8 (−25%, P < 0.05) and tumor necrosis factor α (−20%, P < 0.05), either in basal conditions or after a lipopolysaccharide challenge. By contrast, the gut structure, barrier function (measured ex vivo in Ussing chambers), microbial diversity (assessed by 16S rRNA pyrosequencing), and colonic short-chain fatty acid content were unchanged, assuming maintenance of normal intestinal physiology. In conclusion, this work confirms in vivo probiotic properties of dairy propionibacteria-fermented products, which are promising for the prevention or healing of inflammatory bowel diseases.
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