Identification of Resistance QTLs to Black Leaf Streak Disease (Due to Pseudocercospora fijiensis) in Diploid Bananas (Musa acuminata)
2024
Carreel, Françoise | Martin, Guillaume | Ravel, Sébastien | Roussel, Véronique | Pages, Christine | Habas, Rémy | Cantagrel, Théo | Guiougou, Chantal | Delos, Jean-Marie | Hervouet, Catherine | Mournet, Pierre | d'Hont, Angélique | Yahiaoui, Nabila | Salmon, Frédéric | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) | Département Systèmes Biologiques (Cirad-BIOS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Plant Health Institute of Montpellier (UMR PHIM) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) | This study was funded by the 2018 ‘DUREBAN’ project financed by the French Biodiversity Office as part of the “Seeds” call for research projects co-sponsored by the special allocation account for agricultural and rural development (CASDAR) and the research and innovation axis of the Écophyto II+ plan managed by the French Ministry of Ecological Transition and Territorial Cohesion (French acronym MTECT), Ministry of Agriculture and Food Sovereignty (French acronym MASA), Ministry of Health and Prevention (French acronym MSP) and the Ministry of Higher Education and Research (French acronym MESR). The work has also been realized with the support of MESO@LR-Platform at the University of Montpellier, the financial support of CIRAD, and the technical support of the bioinformatics group of the UMR AGAP Institute, member of the French Institute of Bioinformatics (IFB)—South Green Bioinformatics Platform. | MESO@LR-Platform, University of Montpellier, France | South Green Bioinformatics Platform, Montpellier, France
The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/horticulturae10060608/s1, Figure S1: Location of the recombinations detected on each parent (KNO and IDN110) on the physical map of DH Pahang reference genome version 4 [37]; Figure S2: Manhattan plots of KNO (a.) and IDN110 (b.) for the 16 phenotypic variables; Table S1: For all hybrids, BLUP values of the phenotypic variables and genotypes for the 2084 non-redundant markers; Table S2: List of the genes or putative genes identified in the four main QTLs of IDN110.
显示更多 [+] 显示较少 [-]International audience
显示更多 [+] 显示较少 [-]英语. Black Leaf Streak Disease (BLSD), caused by the fungus Pseudocercospora fijiensis, is a recent pandemic and the most economically and environmentally important leaf disease of banana. To assist breeding of varieties with durable resistance to the rapidly evolving P. fijiensis, we used a diploid genitor 'IDN 110' with partial resistance to BLSD to search for QTLs. We assessed diploid progeny of 73 hybrids between 'IDN 110' and the diploid cultivar 'Khai Nai On', which is susceptible to BLSD. Hybrids were phenotyped with artificial inoculation under controlled conditions. This method allowed us to focus on resistance in the early stages of the interaction already identified as strongly influencing BLSD epidemiology. Progeny were genotyped by sequencing. As both parents are heterozygous for large reciprocal translocations, the distribution of recombination was assessed and revealed regions with low recombination rates. Fourteen non-overlapping QTLs of resistance to BLSD were identified of which four main QTLs from the 'IDN110' parent, located on chromosomes 06, 07, 08, and 09, were shown to be of interest for marker-assisted selection. Genes that underline those four QTLs are discussed in the light of previous literature.
显示更多 [+] 显示较少 [-]