Development of genomic probes for detection of hepatitis A virus and enteroviruses in shellfish

Apaire, M; Leguyader, F; Besse, B; Billaudel, S

Development of genomic probes for detection of hepatitis A virus and enteroviruses in shellfish

1992

Apaire, M | Leguyader, F | Besse, B | Billaudel, S

The development of molecular biology techniques has modified research procedures for certain viruses, particularly hepatitis A virus (HAV) and enteroviruses. Accordingly, we designed cDNA and RNA genomic probes to detect viruses in shellfish in the environment. Better sensitivity was noted with RNA than cDNA probes: only 17/83 shellfish samples were positive with HAV and enterovirus DNA probes, where as 42/83 were positive with HAV and enterovirus RNA probes.

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