Deconjugation and bile salts hydrolase activity by Bifidobacterium strains with acquired resistance to bile
2005
Noriega Pérez, Luis | Cuevas, Isabel | Margolles Barros, Abelardo | González de los Reyes-Gavilán, Clara | European Commission | Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Deconjugation by bile salts hydrolases in probiotics has been related to reduction of serum cholesterol levels in mammals. We compared the susceptibility to conjugated primary (glycocholate and taurocholate) and secondary (glycodeoxycholate and taurodeoxycholate) salts and the level of hydrolase activity of Bifidobacterium strains with acquired resistance to bile and of their more sensitive original strains. Minimum inhibitory concentrations against conjugated salts of the resistant strains were higher than that of the corresponding originals. None of the strains displayed deconjugation against primary salts, whereas most of them deconjugated secondary salts. Salts of cholic acid were more toxic than that of deoxycholic acid. Derivatives showed higher hydrolase activity than their originals. These results suggested a relationship between bile resistance and deconjugation. Finally, the resistance of bifidobacteria against glycodeoxycholate increased in the presence of maltose and cellobiose as compared with glucose, which could be related to a more efficient energy procurement from disaccharides
显示更多 [+] 显示较少 [-]This work was financially supported by Plan Nacional de I + D and European Union FEDER funds (projects AGL2001-2296, AGL2004-06088 and AGL2004-06726).
显示更多 [+] 显示较少 [-]Peer reviewed
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