In vitro acetylcholinesterase inhibitory activity and chemical composition of the dichloromethane extracts of the leaves and inflorescences of the tumbleweed, Trachyandra laxa (N.E.Br.) Oberm. var. rigida (Suesseng.) Roessl
2022
Van Wyk, Hedwig M | Schoeman, Dewald | Kwembeya, Ezekeil
Trachyandra laxa, which belongs to the family Asphodelaceae, is a poisonous geophyte, often consumed by livestock, particularly when pastures are poor. Ingestion of T. laxa by livestock causes paresis and paralysis of the animals. However, the chemical composition of this plant has never been studied before and its toxic components are still unknown. In this study, an acetylcholinesterase (AChE) inhibition assay of different extracts of the parts of Trachyandra laxa (N.E.Br.) Oberm. var. rigida (Suesseng.) Roessl. revealed that the DCM extracts possess the highest AChE inhibitory activity. In addition, using qualitative phytochemical tests, it was determined that all the plant parts contain polyphenols and tannins, while only the leaves contain flavonoids. Saponins were detected in all plant parts, except in the flowers and anthraquinones were detected in all plant parts expect the roots. Using GC MS analysis, 13 volatile constituents were identified in the DCM extracts of the leaves and inflorescences, including a,b-unsaturated lactones, carboxylic acids and esters, a diterpenoid, phytol, and a phytosterol, b-sitosterol. Of particular interest are the a,b-unsaturated lactones, which were identified as dihydroactinidiolide (DHA) and its hydroxylated analogue, hydroxy‑DHA, as well as phytol, a diterpenoid. DHA and phytol have been reported to possess potent AChE inhibition activity. It is therefore plausible that these compounds and potentially also hydroxy‑DHA could contribute to the AChE inhibitory activity of the DCM extracts, and thus the neurotoxicity of T. laxa var. rigida.
显示更多 [+] 显示较少 [-]