Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle
2005
Kaba, S.A. | Musoke, A.J. | Schaap, D. | Schetters, T. | Rowlands, G.J. | Vermeulen, A.N. | Nene, Vishvanath M. | Vlak, J.M. | Oers, M.M. van
Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and the baculovirus envelope protein GP64. Both GFP:p67DeltaSS and GP64:p67C induced antibodies with high ELISA titers that neutralized T. parva sporozoites with high efficiency. Upon challenge, a correlation was observed between the in vitro neutralizing capacity and the reduction in severe ECF for individual animals. A protection level upto 85% was obtained. This level of protection was achieved with only two inoculations of 100mug per dose, which is a major improvement over previous recombinant p67 products.
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