Biofortified indica rice attains iron and zinc nutrition dietary targets in the field
2016
Trijatmiko, Kurniawan R | Dueñas, Conrado | Tsakirpaloglou, Nikolaos | Torrizo, Lina | Mae Arines, Felichi | Adeva, Cheryl | Balindong, Jeanette | Oliva, Norman | Sapasap, Maria V | Borrero Correa, Jaime C. | Rey, Jessica | Francisco, Perigio | Nelson, Andrew | Nakanishi, Hiromi | Lombi, Enzo | Tako, Elad | Glahn, Raymond P. | Stangoulis, James C.R. | Chadha-Mohanty, Prabhjit | Johnson, Alexander A.T. | Tohme, Joseph M. | Barry, Gerard | Slamet-Loedin, Inez H.
More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 μg g−1 iron (Fe) and 16 μg g−1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 μg g−1 Fe and 28 μg g−1 Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 μg g−1 in glasshouse settings; in contrast, under field conditions, 4 μg g−1 was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 μg g−1 Fe and 45.7 μg g−1 Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds.
显示更多 [+] 显示较少 [-]