Lipid profiling and corresponding biodiesel quality of Mortierella isabellina using different drying and extraction methods | Bioresource Technology
2014
Hussain, Javid | Ruan, Zhenhua | Nascimento, Iracema Andrade | Liu, Yan | Liao, Wei | Hussain, Javid | Ruan, Zhenhua | Nascimento, Iracema Andrade | Liu, Yan | Liao, Wei
Texto completo: acesso restrito. p.768–772
显示更多 [+] 显示较少 [-]Four lipid extraction methods (Bligh & Dyer, hexane & isopropanol, dichloromethane & methanol, and hexane) were evaluated to extract lipid from freeze- and oven-dried fungus Mortierella isabellina ATCC42613. The highest lipid yield (41.8%) was obtained from Bligh & Dyer extraction on the oven-dried fungal biomass with a methanol:chloroform:water ratio of 2:1:0.8. Other lipid extraction methods on both freeze- and oven-dried samples had lipid yields ranging from 20.7% to 35.9%. Non-polar lipid was the main lipid class (more than 90% of total lipid) in M. isabellina. Regarding fatty acid profile, there was no significant difference on fatty acid concentration between different drying and extraction methods. Estimation of biodiesel fuel properties using correlative models further demonstrated that the fungal biodiesel is a good alternative to fossil diesel.
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