The separation and identification of picomole amounts of intermediates of glucose metabolism by high performance liquid chromatography on pellicular resins
1992
Preller Simmons, Ana | Guixé Leguía, Victoria Cristina | Torres, Juan Carlos | Hofer, Hans | Ureta Aravena, Tito
A column (CarboPac PA1, Dionex) containing an anion-exchange pellicular resin was used for the separation of phosphoryl-hexoses derived from labeled glucose microinjected into individual frog oocytes or from cultures of Escherichia coli. Intermediates were identified by: a) comparison of retention times with those of authentic commercial compounds; b) the use of internal labeled standards; c) incubation of samples with specific enzymes and noting the disappearance of one radioactive peak and appearance of another at a new retention time.
显示更多 [+] 显示较少 [-]