Detection of Haplosporidium pinnae from Pinna nobilis Faeces
2022
Lopez-Nuñez, Raquel | Cortés Melendreras, Emilio | Gimenez-Casalduero, Francisca | Prado, Patricia | Lopez-Moya, Federico | Lopez-Llorca, Luis Vicente | Lopez-Llorca, Luis Vicente | Lopez-Moya, Federico | Gimenez-Casalduero, Francisca | Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef" | Biología Marina | Fitopatología
Pinna nobilis (Linnaeus, 1758) is the largest bivalve endemic to the Mediterranean. It is distributed in a wide range of coastal environments, including estuaries. Pinna nobilis has recently become a critically endangered species (with almost 100% mortality) along the entire Spanish Mediterranean coast. This may be due to coinfections caused by Haplosporidium pinnae and bacterial pathogens such as Mycobacterium spp. We extensively sampled P. nobilis from Mar Menor lagoon (SE Spain), a site where individuals still survive. Using conventional PCR, we found Haplosporidium spp. in 7.1% of mantle and faecal DNA samples in different individuals of P. nobilis. We identified and quantified Haplosporidium pinnae in P. nobilis using Sanger sequencing and qPCR. Faecal H. pinnae detection is non-invasive, unlike biopsies. Therefore, this non-lethal and non-invasive sampling method could contribute to the welfare of living populations, particularly in eutrophic environments, where they are prone to septicaemia. The use of faecal DNA analysis could be a major advance in epidemiology and recovery assessment studies of P. nobilis.
显示更多 [+] 显示较少 [-]This research was funded by the project DGMARMENOR-MURCIA1-21T entitled: Study, Evaluation and Molecular Detection of Pathogenic Haplosporidium pinnae in Species of Pinna nobilis from Mar Menor and the Department of Marine Science and Applied Biology, the University of Alicante. Fondo Europeo de Desarrollo Regional Una manera de hacer Europa.
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