Seroprevalencia de Brucella spp. en cerdo de abasto en el Camal Municipal del Cantón Babahoyo

Porcine brucellosis is a disease caused by the bacteria brucella suis, which causes reproductive losses. The main objective of this research was to determine the seroprevalence of Brucella spp. in pigs for supply in the Municipal Camal of the Babahoyo Canton, with sample numbers of 267 pigs from different sectors that arrive at the slaughter center, there was the presence of a total of females (n=152), and a total of males (n=115) destined for sacrifice. The Rose Bengal (RB) Seroagglutination test was performed, sampling was demonstrated from the pigs before being slaughtered using the venipuncture technique in the jugular vein, blood samples were extracted and transferred to a refrigerator at a temperature of 4°. C to obtain the serum, in the Pathophysiology Laboratory of the Faculty of Agricultural Sciences of the Technical University of Babahoyo, to proceed with the rose bengal diagnostic technique, 0.30ul of the reagent (Rose Bengal) and 0.30ul were used. of serum on a glass plate to homogenize them, wait around 4 minutes making circular movements, after this the diagnosis is read directly with light, when diagnosing the results were 0% negative in Brucella spp, finding a low percentage of animals carriers of antibodies, in conclusion it was determined that brucellosis in the slaughterhouse was not diagnosed with positive cases, it is unusual with positive tests present in the samples studied in other slaughter centers.

Porcine brucellosis is a disease caused by the bacteria brucella suis, which causes reproductive losses. The main objective of this research was to determine the seroprevalence of Brucella spp. in pigs for supply in the Municipal Camal of the Babahoyo Canton, with sample numbers of 267 pigs from different sectors that arrive at the slaughter center, there was the presence of a total of females (n=152), and a total of males (n=115) destined for sacrifice. The Rose Bengal (RB) Seroagglutination test was performed, sampling was demonstrated from the pigs before being slaughtered using the venipuncture technique in the jugular vein, blood samples were extracted and transferred to a refrigerator at a temperature of 4°. C to obtain the serum, in the Pathophysiology Laboratory of the Faculty of Agricultural Sciences of the Technical University of Babahoyo, to proceed with the rose bengal diagnostic technique, 0.30ul of the reagent (Rose Bengal) and 0.30ul were used. of serum on a glass plate to homogenize them, wait around 4 minutes making circular movements, after this the diagnosis is read directly with light, when diagnosing the results were 0% negative in Brucella spp, finding a low percentage of animals carriers of antibodies, in conclusion it was determined that brucellosis in the slaughterhouse was not diagnosed with positive cases, it is unusual with positive tests present in the samples studied in other slaughter centers.

Brucelosis porcina es una enfermedad causada por la bacteria brucella suis provoca pérdidas reproductivas, la presente investigación tuvo como objetivo principal, Determinar la Seroprevalencia de Brucella spp. en cerdo de abasto en el Camal Municipal del Cantón Babahoyo, con números de muestras de 267 porcinos de diferentes sectores que llegan al centro de faenamiento, se contó con la presencia de un total en hembras (n=152), y un total de machos (n=115) destinados al sacrificio. Se realizó la prueba Seroaglutinación Rosa de Bengala (RB), se consideró la toma de muestras a los porcinos antes de ser sacrificados mediante la técnica de venopunción en la vena yugular se extrajo muestra sanguínea las misma que fueron trasladadas en cuoler a temperatura de 4 °C para la obtención del suero, en el Laboratorio de Fisiopatología de la Facultad de Ciencias Agropecuarias de la Universidad Técnica de Babahoyo, para proceder con la técnica de diagnóstico rosa de bengala, se utilizó 0.30ul del reactivo (Rosa de Bengala) y 0.30ul de suero en una placa de vidrio para homogenizarlos, esperar alrededor de 4 minutos haciendo movimientos circulares, luego de esto se da lectura del diagnóstico con luz de manera directa, al diagnosticar dieron como resultados 0% negativos en Brucella spp encontrándose un bajo porcentaje de animales portadores de anticuerpos, en conclusión se llegó a determinar que la brucelosis en el camal no fue diagnosticada con casos positivos, es inusual con pruebas positivas presentes de las muestras estudiadas en otros centros de faenamientos.