The developmental regulator SEEDSTICK controls structural and mechanical properties of the arabidopsis seed coat
2016
Ezquer, Ignacio | Mizzotti, Chiara | Nguema-Ona, Eric | Gotté, Maxime | Beauzamy, Léna | Viana, Vivian Ebeling | Dubrulle, Nelly | Costa de Oliveira, Antonio | Caporali, Elisabetta | Koroney, Abdoul-Salam | Boudaoud, Arezki | Driouich, Azeddine | Colombo, Lucia | Università degli Studi di Milano = University of Milan (UNIMI) | Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV) ; Université de Rouen Normandie (UNIROUEN) ; Normandie Université (NU)-Normandie Université (NU) | Reproduction et développement des plantes (RDP) ; École normale supérieure de Lyon (ENS de Lyon) ; Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Centre National de la Recherche Scientifique (CNRS) | Universidade Federal de Pelotas = Federal University of Pelotas (UFPel)
International audience
显示更多 [+] 显示较少 [-]英语. Although many transcription factors involved in cell wall morphogenesis have been identified and studied, it is still unknown how genetic and molecular regulation of cell wall biosynthesis is integrated into developmental programs. We demonstrate by molecular genetic studies that SEEDSTICK (STK), a transcription factor controlling ovule and seed integument identity, directly regulates PMEI6 and other genes involved in the biogenesis of the cellulose-pectin matrix of the cell wall. Based on atomic force microscopy, immunocytochemistry, and chemical analyses, we propose that structural modifications of the cell wall matrix in the stk mutant contribute to defects in mucilage release and seed germination under water-stress conditions. Our studies reveal a molecular network controlled by STK that regulates cell wall properties of the seed coat, demonstrating that developmental regulators controlling organ identity also coordinate specific aspects of cell wall characteristics.
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