2,4-D induction of somaclonal variations in in vitro grown date palm (Phoenix dactylifera L. cv Barhee)
2022
Baklouti, Emna | Beulé, Thierry | Nasri, Ameni | Ben Romdhane, Amal | Drira, Riadh | Doulbeau, Sylvie | Rival, Alain | Drira, Noureddine | Fki, Lotfi | جامعة صفاقس - Université de Sfax - University of Sfax | Laboratory of Plant Biotechnology [Sfax] ; Faculté des Sciences de Sfax (FSS) ; جامعة صفاقس - Université de Sfax - University of Sfax-جامعة صفاقس - Université de Sfax - University of Sfax | Diversité, adaptation, développement des plantes (UMR DIADE) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [Occitanie])-Université de Montpellier (UM) | Département Systèmes Biologiques (Cirad-BIOS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Green Biotechnology LtD | Agrosystèmes Biodiversifiés (UMR ABSys) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre International de Hautes Etudes Agronomiques Méditerranéennes - Institut Agronomique Méditerranéen de Montpellier (CIHEAM-IAMM) ; Centre International de Hautes Études Agronomiques Méditerranéennes (CIHEAM)-Centre International de Hautes Études Agronomiques Méditerranéennes (CIHEAM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) | Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Our research work was undertaken at the Faculty of Sciences of Sfax, Tunisia. The present research was partly implemented at the Institute of Research for Development (IRD) at UMR-DIADE in Montpellier, France. The authors would like to thank the COST office (FA1405 coordinator) for financial support. COST is an EU-funded program that enables researchers to set up their interdisciplinary research networks in Europe and beyond.
International audience
显示更多 [+] 显示较少 [-]英语. Key message: Results demonstrate that the 2,4-D can affect physiological and molecular parameters of vitrocultures when used at high concentrations without hampering their morphogenetic capacities. It was found to be efficient at inducing genetic and epigenetic variations.Abstract: The present study is a part of a program designed at improving the date palm, Phoenix dactylifera L. cv. Barhee, through induced somaclonal variation. In this work, caulogenic cultures were subcultured on MS media supplemented with 0, 1, 5, 10, 20 and 40 mg L-1 2,4-D in order to induce genetic and epigenetic variations. The highest doses of 2,4-D were found to induce severe negative effects on in vitro cultures, although some tissues were able to survive and to produce calli with high morphogenetic capacities. Our analysis showed some significant effect of 2,4-D on several physiological parameters. Indeed, chlorophyll and growth rates were found to drastically decrease while proline content increased from 535 to 2973 nmol g(-1) FW when 40 mg L-1 2,4-D were used. In vitro cultures showed several signs of oxidative stress, such as high levels of hydrogen peroxide and malondialdehyde; likewise, the specific activity of several antioxidant enzyme was found to increase. Plant regeneration from in vitro cultures treated with 2,4-D was obtained after subculturing explants onto PGR-free media. The ISSR analysis of 2,4-D-treated material showed that this plant growth regulator (PGR) induced measurable genetic variations. The global DNA methylation rates (GMR) as estimated through the HPLC analysis of nucleosides also confirmed the presence of epigenetic changes caused by 2,4-D as GMRs increased from 13.8 to 18.93%.
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